Active Recombinant Human TNFSF13B protein

• Cat.No.: TNFSF13B-2571H
• Product Overview: Recombinant Human TNFSF13B protein(Ala134-Leu285) was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 134-285 aa
• Form: Lyophilized from a 0.22 μm filtered solution of 50mM Tris, 150mM NaCl, pH 8.0.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human BAFF at 5 μg/mL (100 μL/well) can bind recombinant human BCMA with a linear range of 3-20 ng/mL. 2. Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED50 for this effect is 0.1-1 ng/mL, corresponding to a specific activity of 1×10^6-1×10^7 units/mg. 3. Loaded Human TNFRSF17/BCMA/CD269 Protein, C-hFc&His on ProA Biosensor, can bind Human TNFSF13B/BAFF/CD257 Protein, no Tag with an affinity constant of 3.05nM as determined in BLI assay (Gator).
• Molecular Mass: 17.04 kDa
• Endotoxin: Please contact us for more information.
• Purity: ≥ 90 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: TNFSF13B tumor necrosis factor (ligand) superfamily, member 13b [ Homo sapiens ]
• Official Symbol: TNFSF13B
• Synonyms: TNFSF13B; tumor necrosis factor (ligand) superfamily, member 13b; TNFSF20; tumor necrosis factor ligand superfamily member 13B; BAFF; BLYS; CD257; TALL 1; TALL1; THANK; delta BAFF; Delta4 BAFF; B-lymphocyte stimulator; B-cell-activating factor; ApoL related ligand TALL-1; TNF homolog that activates apoptosis; dendritic cell-derived TNF-like molecule; tumor necrosis factor-like protein ZTNF4; TNF and ApoL-related leukocyte expressed ligand 1; tumor necrosis factor (ligand) superfamily, member 20; DTL; ZTNF4; TALL-1
• Gene ID: 10673
• mRNA Refseq: NM_001145645
• Protein Refseq: NP_001139117
• MIM: 603969
• UniProt ID: Q9Y275