Active Recombinant Human TNNT2 protein, His-GST-tagged

• Cat.No.: TNNT2-3327H
• Product Overview: Recombinant Human TNNT2 protein(Met1~Lys295), fused with N-terminal His and GST Tag, was expressed in E.coli.

Specification

• Source: E.coli
• Species: Human
• Tag: His-GST
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Bio-activity: Troponin T Type 2, Cardiac (TNNT2) is one of three troponin isoforms found in the tropomyosin-troponin complex. This complex is responsible for the calcium sensitivity of the contractile apparatus in the muscle. Cardiac Troponin T is used as a biological marker for cardiomyocytes and its level in serum is frequently used as an indicator of myocardial cell injury. Besides, Troponin I Type 3, Cardiac (TNNI3) has been identified as an interactor of TNNT2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TNNT2 and recombinant human TNNTI3. Briefly, TNNT2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to TNNI3-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TNNT2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450/630nm immediately. The binding activity of TNNT2 and TNNI3 was shown in Figure 1, and this effect was in a dose dependent manner.
• Molecular Mass: 70kDa
• Protein Length: Met1~Lys295
• Purity: > 95%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: TNNT2 troponin T type 2 (cardiac) [ Homo sapiens ]
• Official Symbol: TNNT2
• Synonyms: TNNT2; troponin T type 2 (cardiac); cardiomyopathy, hypertrophic 2 , CMH2, troponin T2, cardiac; troponin T, cardiac muscle; troponin T2, cardiac; cardiac muscle troponin T; cardiomyopathy, hypertrophic 2; cardiomyopathy, dilated 1D (autosomal dominant); CMH2; RCM3; TnTC; cTnT; CMPD2; LVNC6; MGC3889
• Gene ID: 7139
• mRNA Refseq: NM_000364
• Protein Refseq: NP_000355
• MIM: 191045
• UniProt ID: P45379