Active Recombinant Human TSHR protein, Fc-tagged

• Cat.No.: TSHR-1856H
• Product Overview: Recombinant Human TSHR(Met22-Gly413) fused with Fc of Human IgG1 at C-terminal was expressed in CHO.

Specification

• Species: Human
• Source: CHO
• Tag: Fc
• Protein Length: 22-413 a.a.
• Predicted N Terminal: Met22
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit rhTSH-induced cAMP accumulation in HEK293 human embryonic kidney cells transfected with human TSH R. The ED50 for this effect is 2-12 μg/mL.
• Molecular Mass: 71 kDa
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.

Gene Information

• Gene Name: TSHR thyroid stimulating hormone receptor [ Homo sapiens ]
• Official Symbol: TSHR
• Synonyms: TSHR; thyroid stimulating hormone receptor; thyrotropin receptor; LGR3; TSH-R; thyrotropin receptor-I, hTSHR-I; seven transmembrane helix receptor; thyroid-stimulating hormone receptor; thyroid stimulating hormone receptor, isoform 2; CHNG1; hTSHR-I; MGC75129
• Gene ID: 7253
• mRNA Refseq: NM_000369
• Protein Refseq: NP_000360
• UniProt ID: P16473