Active Recombinant Human TTK, GST-tagged

• Cat.No.: TTK-1503H
• Product Overview: Recombinant full-length human TTK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: TTK is a serine/threonine kinase that has been implicated in the regulation of centrosome duplication and mitotic checkpoint response. Overexpressing of dominant-negative TTK in human cell lines prevents centrosome duplication while active TTK accelerates centrosome reduplication in an osteosarcoma cell line. Disruption of TTK function leads to a combination of severe mitotic abnormalities and failure in centrosome duplication. TTK is required for stabilization and activation of p53 during spindle disruption. TTK phoshorylates the N-terminal domain of p53 at Thr18, and this phosphorylation disrupts the interaction with MDM2 and abrogates MDM2-mediated p53 ubiquitination.
• Source: Sf9 Cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 19 nmol/min/mg
• Molecular Mass: ~130 kDa
• Protein length: Full length
• Purity: >75%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: TTK TTK protein kinase [ Homo sapiens ]
• Official Symbol: TTK
• Synonyms: TTK; TTK protein kinase; dual specificity protein kinase TTK; cancer/testis antigen 96; CT96; MPS1L1; monopolar spindle 1-like 1; phosphotyrosine picked threonine kinase; phosphotyrosine picked threonine-protein kinase; ESK; PYT; MPS1; FLJ38280
• Gene ID: 7272
• mRNA Refseq: NM_003318
• Protein Refseq: NP_003309
• MIM: 604092
• UniProt ID: P33981
• Chromosome Location: 6q13-q21
• Pathway: Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem
• Function: ATP binding; binding; nucleotide binding; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; protein tyrosine kinase activity