Active Recombinant Human UBE2A Protein, His-tagged

• Cat.No.: UBE2A-530H
• Product Overview: Recombinant Human UBE2A (Accession # NP_003327), fused with an N-terminal 6-His tag, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: X mg/ml (XµM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
• Bio-activity: Recombinant Human His6-HR6A/UBE2A is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-HR6A/UBE2A concentration of 0.1-1 µM.
• Molecular Mass: Recombinant Human UBE2A, His-tagged has a calculated MW of 19 kDa.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 10 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: UBE2A ubiquitin-conjugating enzyme E2A [ Homo sapiens ]
• Official Symbol: UBE2A
• Synonyms: UBE2A; ubiquitin-conjugating enzyme E2A; ubiquitin conjugating enzyme E2A (RAD6 homolog); ubiquitin-conjugating enzyme E2 A; HHR6A; RAD6A; UBC2; HR6A; RAD6 homolog A; ubiquitin-protein ligase A; ubiquitin carrier protein A; ubiquitin-conjugating enzyme E2A (RAD6 homolog); MRXSN; MRXS30
• Gene ID: 7319
• mRNA Refseq: NM_003336
• Protein Refseq: NP_003327
• MIM: 312180
• UniProt ID: P49459
• Chromosome Location: Xq24