Recombinant Human UBE2A, His-tagged

• Cat.No.: UBE2A-53H
• Product Overview: Recombinant human UBE2A, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 19.4 kDa (172aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Ubiquitin-conjugating enzyme E2 A isoform 1, also known as UBE2A is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Multiple alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 1mM EDTA.
• Purity: > 95% by SDS - PAGE.
• Concentration: 0.5mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MSTPARRRLM RDFKRLQEDP PAGVSGAPSE NNIMVWNAVI FGPEGTPFED GTFKLTIEFT EEYPNKPPTV RFVSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWR DC.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: UBE2A ubiquitin-conjugating enzyme E2A (RAD6 homolog) [ Homo sapiens ]
• Synonyms: ubiquitin-conjugating enzyme E2A (RAD6 homolog); UBC2; HHR6A; RAD6A; UBE2A; ubiquitin-conjugating enzyme E2 A; HR6A; RAD6 homolog A; ubiquitin-protein ligase A; ubiquitin carrier protein A; Ubiquitin-protein ligase A; Ubiquitin carrier protein A; EC 6.3.2.19; hHR6A
• Gene ID: 7319
• mRNA Refseq: NM_003336
• Protein Refseq: NP_003327
• MIM: 312180
• UniProt ID: P49459
• Chromosome Location: Xq24-q25
• Pathway: Ubiquitin mediated proteolysis
• Function: ATP binding; ligase activity; nucleotide binding; protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity