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Active Recombinant Human UBE2F Protein, His-tagged

Cat.No. : UBE2F-803H
Product Overview : Recombinant Human UBE2F (Accession # NP_542409), fused with an N-terminal 6-His tag, was produced in E. coli.
  • Specification
  • Gene Information
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Source : E. coli
Species : Human
Tag : His
Form : X mg/ml (X µM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Bio-activity : Recombinant Human His6-UBE2F is a member of the NEDD8-conjugating (E2) enzyme family that receives NEDD8 from a NEDD8-activating (E1) enzyme and subsequently interacts with a NEDD8 ligase (E3) to conjugate NEDD8 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2F concentration of 0.1-1 µM.
Molecular Mass : Recombinant Human UBE2F, His-tagged has a calculated MW of 23 kDa.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution : Reconstitute at 50 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name : UBE2F ubiquitin-conjugating enzyme E2F (UBC6 homolog, C. elegans) [ Homo sapiens ]
Official Symbol : UBE2F
Synonyms : UBE2F; ubiquitin-conjugating enzyme E2F (UBC6 homolog, C. elegans); NCE2; NEDD8 conjugating enzyme;
Gene ID : 23665
mRNA Refseq :
Protein Refseq :
MIM :
UniProt ID : Q969M7
Chromosome Location : 2q37.3

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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