Active Recombinant Human USP1 Protein, His-tagged

• Cat.No.: USP1-1066H
• Product Overview: Recombinant Human USP1 (Accession # O94782), fused with a C-terminal 6-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Source: Baculovirus Sf21 Insect Cells
• Species: Human
• Tag: His
• Form: X mg/ml (X µM) in 50 mM HEPES, pH 7.5, 100 mM NaCl, 10% glycerol, 1 mM TCEP
• Bio-activity: Recombinant Human USP1 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP1 concentration of 0.01-0.5 µM.
• Molecular Mass: Recombinant Human USP1, His-tagged has a calculated MW of 89 kDa.
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. Alternatively, reconstitute at 500 µg/mL in sterile 4 mM HCl containing 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: USP1 ubiquitin specific peptidase 1 [ Homo sapiens ]
• Official Symbol: USP1
• Synonyms: USP1; ubiquitin specific peptidase 1; ubiquitin specific protease 1; ubiquitin carboxyl-terminal hydrolase 1; hUBP; ubiquitin thioesterase 1; deubiquitinating enzyme 1; ubiquitin thiolesterase 1; ubiquitin carboxyl terminal hydrolase 1; ubiquitin specific processing protease 1; ubiquitin-specific-processing protease 1; UBP
• Gene ID: 7398
• mRNA Refseq: NM_001017415
• Protein Refseq: NP_001017415
• MIM: 603478
• UniProt ID: O94782
• Chromosome Location: 1p31.3