Active Recombinant Human USP11 protein, His-tagged

• Cat.No.: USP11-08H
• Product Overview: Recombinant Human USP11 fused with His tag at C-terminal was expressed in Mouse NS0 Cells.

Specification

• Description: Ubiquitin carboxyl-terminal hydrolase 11 (USP11) is a specialized cysteine protease with a predicted molecular weight of 110 kDa. USP11 is a member of the peptidase C19 family and the human protein shares 77% amino acid sequence identity with its mouse ortholog. USP11 plays a role in DNA double-strand break (DSB) repair, and cells are hypersensitive to gamma radiation when USP11 has been knocked down. USP11 overexpression is often observed in colorectal cancer and melanoma and is correlated with poor survival. In these cases USP11 may be driving disease states by stabilizing cIAP2. Interestingly, recent genetic analysis suggests that USP11 was generated during the course of vertebrate evolution by a small-scale duplication of the USP4-encoding region.
• Source: Insect cells
• Species: Human
• Tag: His
• Form: X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
• Bio-activity: Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human USP11 concentration of 10-50 nM when using Ubiquitin-AMC or Ubiquitin-Rhodamine substrates.
• Molecular Mass: 111 kDa
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -70 centigrade as supplied.3 months, -70 centigrade under sterile conditions after opening.

Gene Information

• Gene Name: USP11 ubiquitin specific peptidase 11 [ Homo sapiens ]
• Official Symbol: USP11
• Synonyms: USP11; ubiquitin specific peptidase 11; ubiquitin specific protease 11; ubiquitin carboxyl-terminal hydrolase 11; UHX1; ubiquitin thioesterase 11; deubiquitinating enzyme 11; ubiquitin thiolesterase 11; ubiquitin-specific processing protease 11; ubiquitin-specific-processing protease 11; ubiquitin carboxyl-terminal hydrolase, X-linked
• Gene ID: 8237
• mRNA Refseq: NM_004651
• Protein Refseq: NP_004642
• MIM: 300050
• UniProt ID: P51784
• Chromosome Location: Xp11.23
• Pathway: Association of TriC/CCT with target proteins during biosynthesis, organism-specific biosystem; Chaperonin-mediated protein folding, organism-specific biosystem; Metabolism of proteins, organism-specific biosystem; Protein folding, organism-specific biosystem; TNF-alpha/NF-kB Signaling Pathway, organism-specific biosystem
• Function: cysteine-type endopeptidase activity; peptidase activity; protein binding; ubiquitin thiolesterase activity; ubiquitin-specific protease activity