Active Recombinant Human USP2, GST-tagged

• Cat.No.: USP2-182H
• Product Overview: Recombinant human USP2, isoform 4 (amino acid residues 1-396), fused with N-terminal GST, was expressed in E.coli.

Specification

• Description: USP2 influences the nuclear translocation of NF-κB transcription factors in response to inflammation, injury and other environmental changes. In resting cells, NF-κB is bound to the inhibitory protein IκBα, and therefore maintained in an inactive state in the cytoplasm. The proteasomal degradation of IκBα is prevented by USP2 deubiquitylase activity. This regulates the expression of genes that are involved in immune responses, cell proliferation, differentiation and apoptosis (Metzig et al., 2011).
• Source: E. coli
• Species: Human
• Tag: GST
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: Deubiquitylase Enzyme Assay: The activity of GST-USP2 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of GST-USP2 was compared confirming the deubiquitylating activity of GST-USP2.
• Molecular Mass: ~73 kDa
• Purity: >68% by SDS-PAGE
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: USP2 ubiquitin specific peptidase 2 [ Homo sapiens ]
• Official Symbol: USP2
• Synonyms: USP2; ubiquitin specific peptidase 2; ubiquitin specific protease 2; ubiquitin carboxyl-terminal hydrolase 2; UBP41; ubiquitin thioesterase 2; deubiquitinating enzyme 2; ubiquitin specific protease 9; ubiquitin specific protease 12; 41 kDa ubiquitin-specific protease; ubiquitin-specific-processing protease 2; USP9
• Gene ID: 9099
• mRNA Refseq: NM_001243759
• Protein Refseq: NP_001230688
• MIM: 604725
• UniProt ID: O75604
• Chromosome Location: 11q23.3
• Pathway: TNF-alpha/NF-kB Signaling Pathway, organism-specific biosystem
• Function: cyclin binding; cysteine-type endopeptidase activity; identical protein binding; metal ion binding; peptidase activity; protein binding; ubiquitin protein ligase binding; ubiquitin thiolesterase activity; ubiquitin thiolesterase activity