Active Recombinant Human USP20, GST-tagged
Cat.No. : | USP20-162H |
Product Overview : | Recombinant human USP20 (amino acid residues 1-913), fused with N-terminal GST, was expressed in Insect sf21. |
- Specification
- Gene Information
- Related Products
Description : | There are two main classes of DUB, cysteine proteases and metalloproteases. Ubiquitin specific protease 20 (USP20) is a member of the cysteine protease enzyme family and cloning of the gene was first described by Nagase et al. (1999). USP20 has been shown to specifically deubiquitylate and stabilise Hypoxia-inducible factor (HIF)-1α, increasing expression of HIF-1α targeted genes, such as vascular endothelial growth factor (VEGF) (Li et al., 2005). USP20 also deubiquitylates tumour necrosis receptor-associated factor 6 (TRAF6) and Tax and suppresses interleukin 1β (IL-1β) and Tax-induced NF-κB activation. |
Source : | Insect cells |
Species : | Human |
Tag : | GST |
Form : | 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol |
Bio-activity : | Deubiquitylase Enzyme Assay: The activity of GST-USP20 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of GST-USP20 was compared confirming the deubiquitylating activity of GST-USP20. |
Molecular Mass : | 128.7 kDa |
Purity : | >70% by SDS-PAGE |
Storage : | 12 months at -70°C. Avoid multiple freeze/thaw cycles. |
Concentration : | 0.5 mg/ml |
Gene Name : | USP20 ubiquitin specific peptidase 20 [ Homo sapiens ] |
Official Symbol : | USP20 |
Synonyms : | USP20; ubiquitin specific peptidase 20; ubiquitin specific protease 20; ubiquitin carboxyl-terminal hydrolase 20; KIAA1003; hVDU2; ubiquitin thioesterase 20; deubiquitinating enzyme 20; ubiquitin thiolesterase 20; VHL-interacting deubiquitinating enzyme 2; ubiquitin-specific-processing protease 20; pVHL-interacting deubiquitinating enzyme 2; VDU2; LSFR3A; |
Gene ID : | 10868 |
mRNA Refseq : | NM_001008563 |
Protein Refseq : | NP_001008563 |
UniProt ID : | Q9Y2K6 |
Chromosome Location : | 9q34.2 |
Function : | G-protein coupled receptor binding; cysteine-type endopeptidase activity; metal ion binding; peptidase activity; ubiquitin thiolesterase activity; zinc ion binding; |
Products Types
◆ Recombinant Protein | ||
USP20-9952M | Recombinant Mouse USP20 Protein, His (Fc)-Avi-tagged | +Inquiry |
Usp20-6869M | Recombinant Mouse Usp20 Protein, Myc/DDK-tagged | +Inquiry |
USP20-0363H | Recombinant Human USP20 Protein (K120-V914), Tag Free | +Inquiry |
USP20-2323H | Recombinant Human USP20 Protein, His (Fc)-Avi-tagged | +Inquiry |
USP20-0364H | Recombinant Human USP20 Protein (K120-V914), GST tagged | +Inquiry |
◆ Lysates | ||
USP20-466HCL | Recombinant Human USP20 293 Cell Lysate | +Inquiry |
USP20-467HCL | Recombinant Human USP20 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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