Active Recombinant Human USP20, GST-tagged

• Cat.No.: USP20-162H
• Product Overview: Recombinant human USP20 (amino acid residues 1-913), fused with N-terminal GST, was expressed in Insect sf21.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 1-913 a.a.
• Description: There are two main classes of DUB, cysteine proteases and metalloproteases. Ubiquitin specific protease 20 (USP20) is a member of the cysteine protease enzyme family and cloning of the gene was first described by Nagase et al. (1999). USP20 has been shown to specifically deubiquitylate and stabilise Hypoxia-inducible factor (HIF)-1α, increasing expression of HIF-1α targeted genes, such as vascular endothelial growth factor (VEGF) (Li et al., 2005). USP20 also deubiquitylates tumour necrosis receptor-associated factor 6 (TRAF6) and Tax and suppresses interleukin 1β (IL-1β) and Tax-induced NF-κB activation.
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: Deubiquitylase Enzyme Assay: The activity of GST-USP20 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of GST-USP20 was compared confirming the deubiquitylating activity of GST-USP20.
• Molecular Mass: 128.7 kDa
• Purity: >70% by SDS-PAGE
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: USP20 ubiquitin specific peptidase 20 [ Homo sapiens ]
• Official Symbol: USP20
• Synonyms: USP20; ubiquitin specific peptidase 20; ubiquitin specific protease 20; ubiquitin carboxyl-terminal hydrolase 20; KIAA1003; hVDU2; ubiquitin thioesterase 20; deubiquitinating enzyme 20; ubiquitin thiolesterase 20; VHL-interacting deubiquitinating enzyme 2; ubiquitin-specific-processing protease 20; pVHL-interacting deubiquitinating enzyme 2; VDU2; LSFR3A
• Gene ID: 10868
• mRNA Refseq: NM_001008563
• Protein Refseq: NP_001008563
• UniProt ID: Q9Y2K6
• Chromosome Location: 9q34.2
• Function: G-protein coupled receptor binding; cysteine-type endopeptidase activity; metal ion binding; peptidase activity; ubiquitin thiolesterase activity; zinc ion binding