Active Recombinant Human USP28 protein, His-tagged

• Cat.No.: USP28-23H
• Product Overview: Recombinant Human USP28(Asp155-Ile707) fused with His tag at C-terminal was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 155-707 a.a.
• Description: USP28 is a deubiquitinating enzyme of the C19 peptidase family that regulates MYC stability and DNA damage response checkpoints. USP28 regulates DNA repair by deubiquitinating and thereby stabilizing CLSPN - a protein that is ubiquitinated by the APC/C E3 Ubiquitin ligase in a cell-cycle dependent manner. USP28 interacts with isoform 1 of FBXW7 in the nucleoplasm, and regulates MYC degradation by counteracting its ubiquitination. Recently, a potential role in HIF-1 alpha -dependent processes such as angiogenesis and metastasis has been described for this deubiquitinase. USP28 has been demonstrated to cleave K6, K11, K48, and K63-linked poly-Ubiquitin chains. This recombinant protein contains a C-terminal 6-His tag.
• Form: X mg/ml (X μM) in 50 mM Hepes pH 7.5, 100 mM NaCl, 1 mM TCEP
• Bio-activity: Recombinant Human USP28 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP28 concentration of 10-500 nM.
• Molecular Mass: 123 kDa
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Gene Information

• Gene Name: USP28 ubiquitin specific peptidase 28 [ Homo sapiens ]
• Official Symbol: USP28
• Synonyms: USP28; ubiquitin specific peptidase 28; ubiquitin specific protease 28; ubiquitin carboxyl-terminal hydrolase 28; KIAA1515; ubiquitin thioesterase 28; deubiquitinating enzyme 28; ubiquitin thiolesterase 28; ubiquitin-specific-processing protease 28
• Gene ID: 57646
• mRNA Refseq: NM_020886
• Protein Refseq: NP_065937
• MIM: 610748
• UniProt ID: Q96RU2
• Chromosome Location: 11q23
• Function: cysteine-type peptidase activity; peptidase activity; protein binding; ubiquitin thiolesterase activity; ubiquitin thiolesterase activity; ubiquitin-specific protease activity