USP45-190H
Optional Service: Optional requirements on this protein
Product Overview :
Recombinant human USP45 (amino acid residues 1-814), fused with N-terminal GST, was expressed in E.coli.
Description :
There are two main classes of DUB; cysteine proteases and metalloproteases. Ubiquitin carboxyl-terminal hydrolase 45 (Ubiquitin Specific Protease 45; USP45) is a member of the cysteine protease enzyme family and cloning of the human gene was first described by Quesada et al. (2004). USP45 is an 819 amino acid protein that is broadly expressed; the highest levels can be found in the spleen, ovary and skeletal muscle.
Source :
E. coli
Species :
Human
Tag :
GST
Form :
50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Bio-activity :
Deubiquitylase Enzyme Assay: The activity of GST-USP45 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of GST-USP45 was compared confirming the deubiquitylating activity of GST-USP45.
Molecular Mass :
~118 kDa
Purity :
>70% by SDS-PAGE
Storage :
12 months at -70°C. Avoid multiple freeze/thaw cycles.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.