Active Recombinant Human VCAM1 protein, His-tagged

Cat.No. : VCAM1-170H
Product Overview : Recombinant Human VCAM1(Phe25-Glu698) fused with His tag at the C-terminus was expressed in NS0.
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Source : Mammalian cells
Species : Human
Tag : His
Predicted N Terminal : Met
Form : Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10^4 cells/well are added to human VCAM-1 coated plates (10 µg/mL with 100 µL/well), approximately 90-100% will adhere after 1 hour incubation at RT.Optimal dilutions should be determined by each laboratory for each application.
Molecular Mass : Predicted Molecular Mass: 101.6 (monomer) kDa;SDS-PAGE: 130-140 kDa, reducing conditions
Endotoxin : <0.10 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Storage : Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 100 μg/mL in sterile PBS.
Gene Name : VCAM1 vascular cell adhesion molecule 1 [ Homo sapiens ]
Official Symbol : VCAM1
Synonyms : VCAM1; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; CD106; CD106 antigen; INCAM-100; MGC99561; DKFZp779G2333;
Gene ID : 7412
mRNA Refseq : NM_001078
Protein Refseq : NP_001069
MIM : 192225
UniProt ID : P19320
Chromosome Location : 1p32-p31
Pathway : Adaptive Immune System, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cellular roles of Anthrax toxin, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem;
Function : cell adhesion molecule binding; cell adhesion molecule binding; integrin binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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