Active Recombinant Human VEGF121 protein, Avi-His-tagged, Biotinylated

• Cat.No.: VEGF121-0818H
• Product Overview: Biotinylated Recombinant Human VEGF121 protein(Ala27-Arg147), fused with Avi-His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 27-147 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized VEGFR1/R2-Fc at 1 μg/mL (100 μL/well) can bind Biotinylated Human VEGF121, Avitag,His Tag with a linear range of 0.1-2 ng/mL.
• Molecular Mass: This protein carries an Avi tag (Avitag) at the N-terminus, followed by a polyhistidine tagThe protein has a calculated MW of 16.7 kDa. The protein migrates as 18 kDa and 22 kDa under reducing (R) condition, and 35-40 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ala27-Arg147
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin