Active Recombinant Human VEGFA protein

• Cat.No.: VEGFA-210H
• Product Overview: Active Recombinant Human VEGFA protein(P15692-3) (Met 1-Arg 209) was expressed in Insect Cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Non
• Protein Length: 1-209 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured in a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) (Conn, G. et al. 1990, Proc. Natl. Acad. Sci. USA 87:1323.). The ED50 for this effect is typically 10-50 ng/ml.
• Molecular Mass: The recombinant human VEGF consists of 183 amino acids and has a predicted molecular mass of 21 kDa. The apparent molecular mass of the human VEGF183 is approximately 25-27 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE and SEC-HPLC Analysis.
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: VEGFA vascular endothelial growth factor A [ Homo sapiens ]
• Official Symbol: VEGFA
• Synonyms: VEGFA; vascular endothelial growth factor A; vascular endothelial growth factor, VEGF; VEGF A; VPF; vascular permeability factor; VEGF; MVCD1; MGC70609
• Gene ID: 7422
• mRNA Refseq: NM_001025366
• Protein Refseq: NP_001020537
• UniProt ID: P15692