Active Recombinant Human VEGFA, His-tagged

• Cat.No.: VEGFA-101H
• Product Overview: Human VEGFA (165 a.a.) full-length recombinant protein with His tag expressed in Barley grain (Hordeum vulgare).

Specification

• Species: Human
• Source: Barley Grain
• Tag: His
• Description: This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.
• Form: Lyophilized
• Bio-activity: The ED50 is determined by Cell Proliferation Assay for the dose-dependent effects of serial dilutions of recombinant human VEGFA on HUVEC cells. The ED50 value was determined to be 0.58 ng/mL.
• Molecular Mass: 30 kDa
• Endotoxin: Endotoxin level is less than 0.005ng per ug protein (0.05EU/ug) as measured by turbidimetric kinetic assay
• Purity: >95% by SDS-PAGE
• Applications: Western Blot (Recombinant protein); Functional Study; SDS-PAGE
• Storage: After reconstitution with sterile water to a concentration of no less than 100 ug/mL, store at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Aliquot to avoid repeated freezing and thawing.
• Concentration: 100 ug/mL
• Storage Buffer: Lyophilized from PBS, pH 7.2

Gene Information

• Gene Name: VEGFA vascular endothelial growth factor A [ Homo sapiens(human) ]
• Official Symbol: VEGFA
• Synonyms: VEGFA; VPF; VEGF; MVCD1; vascular endothelial growth factor A; vascular permeability factor
• Gene ID: 7422
• mRNA Refseq: NM_001171626
• Protein Refseq: NP_001165097
• MIM: 192240
• UniProt ID: P15692
• Chromosome Location: 6p12
• Pathway: Angiogenesis; Bladder cancer; Cellular response to hypoxia
• Function: chemoattractant activity; cytokine activity; extracellular matrix binding