Active Recombinant Human VTCN1 Protein, His-tagged

• Cat.No.: VTCN1-755H
• Product Overview: Recombinant Human VTCN1 (Accession # NP_078902) Phe29-Ala258, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Phe29
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes.The ED50 for this effect is typically 0.5 - 2.5 µg/mL.
• Molecular Mass: Recombinant Human VTCN1, His-tagged has a calculated MW of 26.7 kDa. In SDS-PAGE migrates as 45-60 kDa, reducing conditions.
• Protein length: Phe29-Ala258
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 25 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: VTCN1 V-set domain containing T cell activation inhibitor 1 [ Homo sapiens ]
• Official Symbol: VTCN1
• Synonyms: VTCN1; V-set domain containing T cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1; B7 family member; H4; B7 superfamily member 1; B7 H4; B7H4; B7S1; B7X; FLJ22418; B7 family member, H4; T cell costimulatory molecule B7x; T-cell costimulatory molecule B7x; immune costimulatory protein B7-H4; B7-H4; B7h.5; VCTN1; PRO1291; RP11-229A19.4
• Gene ID: 79679
• mRNA Refseq: NM_001253849
• Protein Refseq: NP_001240778
• MIM: 608162
• UniProt ID: Q7Z7D3
• Chromosome Location: 1p12