| Species : |
Human |
| Source : |
Insect Cells |
| Tag : |
His |
| Protein Length : |
215-646 aa |
| Description : |
WEE1 is a serine/threonine protein kinase that acts as a critical regulator of the cell cycle, particularly at the G₂/M checkpoint. It inhibits cell cycle progression by phosphorylating and inactivating CDK1 (Cyclin-Dependent Kinase 1) at Tyr15, thereby preventing premature entry into mitosis and allowing time for DNA repair. |
| Form : |
Supplied as 0.2 μm filtered solution in 50 mM Tris, 300 mM NaCl, pH7.5 with glycerol as protectant. |
| Bio-activity : |
The WEE1 assay is performed using the ADP-Glo TM Kinase Assay kit which quantifies the amount of ADP produced by the WEE1 reaction. The ADP-Glo TM Reagent is added to terminate the kinase reaction and to deplete the remaining ATP, and then the Kinase Detection Reagent is added to convert ADP to ATP and to measure the newly synthesized ATP using luciferase/luciferin reaction. The specific activity is >10 pmol/min/μg. |
| Molecular Mass : |
The protein has a calculated MW of 51.1 kDa. The protein migrates as 8 kDa, 30-33 kDa and 55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Purity : |
>80% as determined by SDS-PAGE. |
| Storage : |
This product is stable after storage at: The product MUST be stored at -70 centigrade or lower upon receipt; -70 centigrade for 3 months under sterile conditions. Please avoid repeated freeze-thaw cycles. |
| Concentration : |
0.2 mg/mL |
| Shipping : |
This product is supplied and shipped with dry ice, please inquire the shipping cost. |
