Active Recombinant Lachnospiraceae bacterium Cas12a protein

• Cat.No.: Cas12a-3070L
• Product Overview: Recombinant Lachnospiraceae bacterium Cas12a protein(Ser 2-His 1228) was expressed in E.coli.

Specification

• Species: Lachnospiraceae
• Source: E.coli
• Tag: Non
• Protein Length: Ser 2-His 1228
• Form: Supplied as 0.2 μm filtered solution in 20 mM NaAC,500 mM NaCl,0.1 mM EDTA,0.1 mM TCEP,pH 6.0 with glycerol as protectant.
• Bio-activity: Measured by its ability to cleave a targeted DNA substrate. Cas12a achieves >90% substrate cleavage.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 147.6 kDa. The protein migrates as 135-145 kDa under reducing (R) condition (SDS-PAGE). This protein has two NLS, which leads to transport to the nucleus efficiently. It is ideal for in vitro and in vivo reactions.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.