Active Recombinant Listeriolysin O, PEST Free

• Cat.No.: hly-243
• Product Overview: Recombinant Listeriolysin O lacking both the signal secretion sequence and the PEST- like sequence is produced in E.Coli. LLO-PEST minus is composed of 471(60-592) amino acids, with the addition of a methionine in its NH2-terminus.

Specification

• Source: E.coli
• Tag: Non
• Protein Length: 60-592 a.a.
• Description: The secreted pore-forming protein listeriolysin O (LLO) is an essential virulence determinant of the facultative intracellular bacterium Listeria monocytogenes that allows the pathogen to escape from the phagocytic vacuole and reach the host cytosol. LLO is a single polypeptide protein encoded by the hlyA gene and composed of 529 residues. At its NH2-terminus it possesses a 25 residues long typical signal sequence excited during the secretion process. Moreover, in its NH2-terminus there is also a 19 amino acids PEST- like sequence that may target this toxin for degradation. This motif seems to be essential for bacterial virulence too, since its deletion causes a defect in bacterium phagosomal escaping.
• Physical Appearance: Sterile Filtered clear solution.
• Formulation: The protein (0.2mg/ml) contains 50mM NaH2PO4, 1mM EDTA, 2.7mM KCl, 1mM DTT, 5% glycerol and 0.5M NaCl.
• Purity: Greater than 90.0% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
• Activity: 1.25x10⁶HU/mg. 2 mM DTT could be use to reactivate the toxin.
• Endotoxin: Less than 0.1ng/µg (IEU/µg) of LLO.
• Applications: a. Cytosolic delivery of molecules, peptides, oligonucleodides and plasmid DNA (4). b. Production of specific monoclonal antibodies (5). c. Detection of anti-listeriolysin O antibodies (6).
• Stability: rLLO although stable at 14C for 1 week, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: hly listeriolysin O [ Listeria monocytogenes str. 4b F2365 ]
• Synonyms: Hly; listeriolysin O; identified by similarity to SP:P13128; match to protein family HMM PF01289
• Gene ID: 2797098
• Protein Refseq: YP_012823
• UniProt ID: Q724L1
• Function: cholesterol binding