Active Recombinant MERS-CoV (NCoV / Novel coronavirus) S Protein, Fc-tagged

• Cat.No.: S-602V
• Product Overview: Recombinant spike protein fragment (Human betacoronavirus 2c EMC/2012)( AFS88936.1)(Cys383-Lys502) was fused with Fc region of mouse IgG at the C-terminus.

Specification

• Species: MERS-CoV
• Source: Insect Cells
• Tag: Fc
• Protein Length: 383-502 a.a.
• Predicted N Terminal: Cys 383
• Form: Lyophilized from sterile 100mM Glycine, 10mM Nacl, pH 7.0, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized Spike (HCoV-EMC/2012) at 10 μg/ml (100 μl/well) can bind biotinylated human ACE2-Fc with a linear range of 0.03-4.0 μg/ml.
• Molecular Mass: The recombinant spike protein fragment (Human betacoronavirus 2c EMC/2012) comprises 354 amino acids and has a predicted molecular mass of 39.7 kDa. It migrates as an approximately 44 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >85 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: S S protein [ Middle East respiratory syndrome coronavirus ]
• Official Symbol: S
• Synonyms: spike glycoprotein; S protein
• Gene ID: 14254594