Active Recombinant Monkey TNFSF10 Protein, Met-tagged, Alexa Fluor 555 conjugated

• Cat.No.: TNFSF10-1191RAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant rhesus TNFSF10 (NP_001252963.1) (Val114-Gly281) was expressed and purified with an initial Met.

Specification

• Species: Monkey
• Source: E.coli
• Protein Length: 169
• Form: Lyophilized
• Bio-activity: 1. Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.5-2 ng/mL.
  2. Measured by its binding ability in a functional ELISA. Immobilized Rhesu
• Molecular Mass: 19.6 kDa
• N-terminal Sequence Analysis: Met
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 98 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, 5%-8% trehalose and mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
• Conjugation: Alexa Fluor 555

Gene Information

• Gene Name: TNFSF10 tumor necrosis factor superfamily member 10 [ Macaca mulatta ]
• Official Symbol: TNFSF10
• Synonyms: tumor necrosis factor (ligand) superfamily, member 10
• Gene ID: 694451
• mRNA Refseq: NM_001266034
• Protein Refseq: NP_001252963