Active Recombinant Monkeypox virus (strain Zaire-96-I-16) A35R protein, His-tagged

• Cat.No.: A35R-16M
• Product Overview: Recombinant Monkeypox virus (strain Zaire-96-I-16) A35R protein(Arg 58 - Thr 181), fused with His tag, was expressed in HEK293.

Specification

• Species: MPX
• Source: HEK293
• Tag: His
• Protein Length: Arg 58 - Thr 181
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Monkeypox virus (strain Zaire-96-I-16) A35R , His Tag at 5 μg/mL (100 μL/well) can bind Anti-Monkeypox-A35R-Antibody with a linear range of 0.1-3 .
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 15.5 kDa. The protein migrates as 15 kDa and 17 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.