Active Recombinant Mouse Ache protein, His-tagged

• Cat.No.: Ache-774M
• Product Overview: Recombinant Mouse Ache protein(Glu 32 - Leu 614), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Glu 32 - Leu 614
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Measured by its ability to cleave Acetylthiocholine. The specific activity is >500 nmol/min/μg, as measured under the described conditions.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 66.7 kDa. The protein migrates as 66-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ache acetylcholinesterase [ Mus musculus ]
• Official Symbol: Ache
• Synonyms: ACHE; acetylcholinesterase; mE1a; mE1b; mE1c; mE1d; mE1e; mE1c-long
• Gene ID: 11423
• mRNA Refseq: NM_009599
• Protein Refseq: NP_033729