Active Recombinant Mouse Acvr2b protein, His-tagged

• Cat.No.: Acvr2b-424M
• Product Overview: Recombinant Mouse Acvr2b protein(NP_031423.1)(Met1-Thr134), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-134 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized Mouse ACVR2B at 2μg/mL (100 μl/well) can bind biotinylated mouse INHBA-His, The EC50 of biotinylated mouse INHBA-His is 40-95 ng/mL.2. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.2-0.8 µg/mL in the presence of 10 ng/mL recombinant Activin A.
• Molecular Mass: The recombinant mouse ACTRIIB/ACVR2B comprises 127 amino acids and has a predicted molecular mass of 14.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmACVR2B is approximately 33-37 kDa due to glycosylation.
• Proteinlength: Met1-Thr134
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 94 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Acvr2b activin receptor IIB [ Mus musculus ]
• Official Symbol: Acvr2b
• Synonyms: ACVR2B; activin receptor IIB; activin receptor type-2B; ACTR-IIB; activin receptor type IIB; ActRIIB
• Gene ID: 11481
• mRNA Refseq: NM_007397
• Protein Refseq: NP_031423