Active Recombinant Mouse Angpt2 protein, His-tagged

• Cat.No.: Angpt2-476M
• Product Overview: Recombinant Mouse Angpt2 protein(NP_031452)(Tyr 19 - Phe 496), fused to C-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Tyr 19 - Phe 496
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human TIE2, Fc Tag at 2 μg/mL (100 μL/well) can bind Mouse Angiopoietin-2, His Tag with a linear range of 2-39 ng/mL.
• Molecular Mass: The protein has a calculated MW of 56.5 kDa. The protein migrates as 60-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.

Gene Information

• Gene Name: Angpt2 angiopoietin 2 [ Mus musculus ]
• Official Symbol: Angpt2
• Synonyms: Angpt2; angiopoietin 2; Ang2; Agpt2; Ang-2; angiopoietin-2
• Gene ID: 11601
• mRNA Refseq: NM_007426
• Protein Refseq: NP_031452
• UniProt ID: O35608