Active Recombinant Mouse Apoa1 protein, hFc-tagged

• Cat.No.: Apoa1-6864M
• Product Overview: Recombinant Mouse Apoa1 protein(Q00623)(Met1-Gln264), fused with hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: hFc
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.2. Immobilized mouse ApoAI at 10 μg/mL (100 μl/well) can bind biotinylated human SCARB1, The EC50 of biotinylated human SCARB1 is 0.27 μg/mL.
• Molecular Mass: The secreted recombinant mouse APOA1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 487 amino acids and has a calculated molecular mass of 55.8 kDa. As a result of glycosylation, the apparent molecular mass of rmAPOA1/Fc monomer is approximately 60 kDa in SDS-PAGE under reducing conditions.
• Protein Length: Met1-Gln264
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Apoa1 apolipoprotein A-I [ Mus musculus ]
• Official Symbol: Apoa1
• Synonyms: APOA1; apolipoprotein A-I; apo-AI; apoA-I; apolipoprotein A1; Sep2; Alp-1; Ltw-1; Sep-1; Sep-2; Apoa-1; Brp-14; Lvtw-1; MGC102525
• Gene ID: 11806
• mRNA Refseq: NM_009692
• Protein Refseq: NP_033822