Active Recombinant Mouse Asah2 protein, His-tagged

• Cat.No.: Asah2-714M
• Product Overview: Recombinant Mouse Asah2 protein(NP_061300.1)(Thr34-Thr756), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Thr34-Thr756
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to hydrolyze the substrate C12:0 ceramide into sphingosine and dodecanoic acid.The specific activity is > 3,000 pmoles/min/μg.
• Molecular Mass: The secreted recombinant mouse ASAH2 consists of 739 amino acids and has a calculated molecular mass of 82 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 105-115 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Asah2 N-acylsphingosine amidohydrolase 2 [ Mus musculus ]
• Official Symbol: Asah2
• Synonyms: ASAH2; N-acylsphingosine amidohydrolase 2; neutral ceramidase; NCDase; N-CDase; acylsphingosine deacylase 2; neutral/alkaline ceramidase; AI585898
• Gene ID: 54447
• mRNA Refseq: NM_018830
• Protein Refseq: NP_061300