Recombinant Human ASAH2 Protein, His&hFc-tagged

• Cat.No.: ASAH2-251H
• Product Overview: Recombinant Human ASAH2 Protein (Phe99-Ile780) with N-His and C-hFc tag was expressed in 293F.

Specification

• Description: Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.
• Source: HEK293F
• Species: Human
• Tag: N-His and C-hFc
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 106.0 kDa
  Accurate Molecular Mass: \
• Protein length: Phe99-Ile780
• Purity: > 80%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: ASAH2 N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 [ Homo sapiens (human) ]
• Official Symbol: ASAH2
• Synonyms: ASAH2; N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2; neutral ceramidase; mitochondrial ceramidase; non-lysosomal ceramidase; acylsphingosine deacylase 2; neutral/alkaline ceramidase; N-acylsphingosine amidohydrolase 2; HNAC1; BCDase; LCDase; NCDase; N-CDase
• Gene ID: 56624
• mRNA Refseq: NM_001143974
• Protein Refseq: NP_001137446
• MIM: 611202
• UniProt ID: Q9NR71