| Description : |
Macrophage Inflammatory Protein-3 beta also called CCL19, ELC (EBI1 Ligand Chemokine), Exodus-3 is a reported β chemokine that binds specifically to the chemokine receptor CCR7/EBI1/BLR2. It is expressed in the thymus, lymph nodes and in activated bone marrow stromal cells. MIP-3 beta is a chemoattractant for T and B lymphocytes and myeloid progenitor cells. |
| Source : |
E. coli |
| Species : |
Mouse |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
Measured by its ability to chemoattract human CCR7 transfected BaF3 mouse proB cells. The ED50 for this effect is typically 3-15 ng/mL. |
| Molecular Mass : |
Approximately 9.2 kDa, a single non-glycosylated polypeptide chain containing 83 amino acids. |
| Protein Length : |
83 |
| AA Sequence : |
GANDAEDCCLSVTQRPIPGNIVKAF RYLLNEDGCRVPAVVFTTLRGYQLC APPDQPWVDRIIRRLKKSSAKNKGN STRRSPVS |
| Endotoxin : |
Less than 1 EU/μg of rMuMIP-3b/CCL19 as determined by LAL method. |
| Purity : |
>95% by SDS-PAGE and HPLC analyses. |
| Storage : |
This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles. |
| Storage Buffer : |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20C. Further dilutions should be made in appropriate buffered solutions. |
