Active Recombinant Mouse Ccl2 Protein (Gln24-Ala127), N-His tagged, Animal-free, Carrier-free

• Cat.No.: Ccl2-75M
• Product Overview: Recombinant Mouse Ccl2 Protein (Gln24-Ala127) with N-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: C-C Motif Chemokine Ligand 2 (CCL2) is a 13.88 kDa cytokine with 125 amino acid residues and is also known as monocyte chemoattractant protein 1 (MCP1). CCL2 is mainly secreted from monocytes, macrophages, and dendritic cells. It regulates many biological functions, such as recruitments of monocytes, memory T cells, eosinophils, and dendritic cells, extravasation of helper T cells, response to TNFα and IFNγ, and angiogenesis. In addition, upon binding with CCR4, it initiates leukocyte migration response to inflammation.
• Source: E. coli
• Species: Mouse
• Tag: N-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to chemoattract BaF3 cells transfected with CCR2A. The ED50 for this effect is < 8 ng/mL.
• Molecular Mass: The protein has a calculated MW of 14.65 kDa.
  The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Gln24-Ala127
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Ccl2 chemokine (C-C motif) ligand 2 [ Mus musculus (house mouse) ]
• Official Symbol: Ccl2
• Synonyms: CCL2; chemokine (C-C motif) ligand 2; C-C motif chemokine 2; small inducible cytokine A2; small-inducible cytokine A2; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; platelet-derived growth factor-inducible protein JE; JE; HC11; MCAF; MCP1; MCP-1; Scya2; Sigje; SMC-CF; AI323594
• Gene ID: 20296
• mRNA Refseq: NM_011333
• Protein Refseq: NP_035463
• UniProt ID: P10148