Active Recombinant Mouse Ccl27a

• Cat.No.: Ccl27a-657M
• Product Overview: Recombinant Mouse Ccl27a (Accession # NP_035466) Leu26-Asn120, fused with , was produced in E. coli.

Specification

• Source: E. coli
• Species: Mouse
• Predicted N Terminal: Leu26
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with mouse CCR10.The ED50 for this effect is typically 0.05-0.25 µg/mL.
• Molecular Mass: Recombinant Mouse Ccl27a has a calculated MW of 10.9 kDa.
• Protein length: Leu26-Asn120
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Tag: Non

Gene Information

• Gene Name: Ccl27a chemokine (C-C motif) ligand 27A [ Mus musculus ]
• Official Symbol: Ccl27a
• Synonyms: CCL27A; chemokine (C-C motif) ligand 27A; C-C motif chemokine 27; skinkine; skinskine; CC chemokine ILC; small inducible cytokine A27; small-inducible cytokine A27; IL-11 R-alpha-locus chemokine; small inducible cytokine A27a; chemokine (C-C motif) ligand 27; cutaneous T-cell-attracting chemokine; ALP; ILC; CTAK; CTACK; Ccl27; PESKY; ESkine; Scya27; Scya27a; AW558992; MGC130150
• Gene ID: 20301
• mRNA Refseq: NM_001048179
• Protein Refseq: NP_001041644

Reviews

09/08/2022

CCL27A has good solubility and ion exchange ability, which is convenient for subsequent experimental treatment and purification.

02/06/2022

The stability of CCL27A has good adaptability under different temperature and pH conditions.

04/29/2021

When conducting experiments with CCL27A, clear and consistent results can be obtained for easy data analysis and interpretation.

Q&As

How can I detect the expression level of CCL27A protein?

Expression level of CCL27A protein can be detected by immunohistochemistry, western blotting, and real-time PCR.

What cell surface receptors does CCL27A protein interact with?

The CCL27A protein interacts with receptors on the surface of skin keratinocytes and immune cells, such as the CCR10 receptor.

What is the interaction between CCL27A protein and other proteins?

Interaction between CCL27A protein and other proteins is regulated by the formation of complexes or the regulation of signal transduction pathways.

How is the expression of CCL27A protein regulated?

The expression of CCL27A protein can be regulated by a variety of factors, including inflammatory factors, ultraviolet light, and infectious agents such as bacteria or viruses.

Can the function of CCL27A protein be studied by overexpression techniques?

Function of the CCL27A protein can be studied by overexpression techniques, such as the use of gene transduction or viral transduction. These techniques can increase the expression level of CCL27A protein to observe its effects on skin inflammation and immune response, among other things.

How can the function of CCL27A protein be studied by gene knockout or knockdown techniques?

The function of the CCL27A protein can be studied by gene knockout or knockdown techniques, such as using techniques such as CRISPR-Cas9 or RNAi. These techniques can create cellular models with missing or reduced CCL27A protein to observe its effects on skin inflammation and immune responses, among other things.