Active Recombinant Mouse CD27 protein, mFc-tagged

• Cat.No.: CD27-2954M
• Product Overview: Recombinant Mouse CD27 protein(Thr 21-Arg 182), fused with mFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: mFc
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse CD27, Mouse IgG2a Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse CD70, His Tag, Avi Tag with a linear range of 1-16 ng/mL.Immobilized Mouse CD27, Mouse IgG2a Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD27 Ligand, Fc Tag with a linear range of 1-16 ng/mL.
• Molecular Mass: This protein carries a mouse IgG2a Fc tag at the C-terminus.The protein has a calculated MW of 45.1 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Thr 21-Arg 182
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd27 CD27 antigen [ Mus musculus ]
• Official Symbol: CD27
• Synonyms: CD27; CD27 antigen; CD antigen 27; CD27L receptor; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7; S152; Tp55; Tnfrsf7
• Gene ID: 21940
• mRNA Refseq: NM_001033126
• Protein Refseq: NP_001028298