Species : |
Mouse |
Source : |
HEK293 |
Tag : |
Avi&Flag&His |
Protein Length : |
Asp 23-Asp 108 (CD3E) & Gln 23-Ser 116 (CD3G) |
Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : |
Immobilized CD3E Monoclonal Antibody (145-2C11) at 1 μg/mL (100 μL/well) can bind Biotinylated Mouse CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag with a linear range of 5-78 ng/mL. |
Molecular Mass : |
Biotinylated Mouse CD3E&CD3G Heterodimer Protein, His,Avitag&Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 16.8 kDa (CD3E) and 15.2 kDa (CD3G). Subunit CD3E carries a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit CD3G is fused with a flag tag at the C-terminus. As a result of glycosylation, the protein migrates as 20-23 kDa under reducing (R) condition, and 35-40 kDa under non-reducing (NR) condition (SDS-PAGE). |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>95% as determined by SDS-PAGE.>90% as determined by SEC-MALS. |
Storage : |
For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |