Recombinant Human CD3E & CD3G cell lysate
Cat.No. : | CD3E & CD3G-761HCL |
Product Overview : | Human CD3E & CD3G Heterodimer derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Tag : | Non |
Gene Name : | CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ]; CD3G CD3g molecule, gamma (CD3-TCR complex) [ Homo sapiens ] |
Official Symbol : | CD3E; CD3G |
Synonyms : | T3E; TCRE; IMD18; T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3; T3G; IMD17; CD3-GAMMA; T-cell surface glycoprotein CD3 gamma chain; T-cell receptor T3 gamma chain; CD3g molecule, epsilon (CD3-TCR complex); CD3g antigen, gamma polypeptide (TiT3 complex); T-cell antigen receptor complex, gamma subunit of T3 |
Gene ID : | 916; 917 |
mRNA Refseq : | NM_000733; NM_000073 |
Protein Refseq : | NP_000724; NP_000064 |
MIM : | 186830; 186740 |
UniProt ID : | P07766; P09693 |
Chromosome Location : | 11q23; 11q23 |
Pathway : | Adaptive Immune System, organism-specific biosystem; Downstream signaling in naive CD8+ T cells, organism-specific biosystem. Adaptive Immune System, organism-specific biosystem; Downstream signaling in naive CD8+ T cells, organism-specific biosystem; HTLV-I infection, conserved biosystem |
Function : | protein binding; receptor signaling protein activity; T cell receptor binding; receptor signaling complex scaffold activity |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (3)
Write a reviewManufacturers with the capacity for bulk production can offer CD3E protein in larger quantities, which is advantageous for researchers working on large-scale experiments or collaborations.
bulk production often leads to economies of scale, making the CD3E protein more cost-effective for research budgets.
Manufacturers can actively seek collaborations and partnerships with researchers to develop new applications or optimize methodologies using CD3E protein.
Q&As (5)
Ask a questionYes, CD3E levels and T-cell function can be monitored to evaluate the response to cancer immunotherapies like checkpoint inhibitors.
There are currently no approved therapies that directly target CD3E, but it is a target for research in developing immunotherapies and cancer treatments.
There may be ongoing clinical trials using CD3E-targeted therapies in the context of cancer and autoimmune diseases. It's important to check clinical trial databases for specific studies.
Yes, monitoring CD3E levels in HIV-infected individuals can provide valuable information about the progression of the disease and the effectiveness of antiretroviral therapy.
CD3E can be used in research to study T-cell responses in allergic conditions, helping to better understand and diagnose allergies.
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