Active Recombinant Mouse CD3E & CD3G protein, His-Flag-tagged, Low Endotoxin

• Cat.No.: CD3E & CD3G-3038M
• Product Overview: Recombinant Mouse CD3E & CD3G protein(P22646(CD3E) & P11942(CD3G))(22-108 aa(CD3E) & 23-116 aa(CD3G)), fused with C-terminal His tag and Flag tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Flag&His
• Protein Length: 22-108 aa(CD3E) & 23-116 aa(CD3G)
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse CD3E&CD3G, His Tag at 1μg/ml(100μl/well) on the plate. Dose response curve for Anti-CD3E Antibody (145-2C11), mFc Tag with the EC50 of 49.4ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 15.34 kDa (CD3E) and 15.73 kDa (CD3G). Due to glycosylation, the protein migrates to 18-23 kDa and 25-27 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Cd3e CD3 antigen, epsilon polypeptide [ Mus musculus ]
• Official Symbol: CD3E
• Synonyms: CD3E; CD3 antigen, epsilon polypeptide; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3; T3e; AI504783; CD3epsilon
• Gene ID: 12501
• mRNA Refseq: NM_007648
• Protein Refseq: NP_031674
• Gene Name 2: Cd3g CD3 antigen, gamma polypeptide [ Mus musculus ]
• Official Symbol 2: CD3G
• Synonyms 2: CD3G; CD3 antigen, gamma polypeptide; T-cell surface glycoprotein CD3 gamma chain; T-cell receptor T3 gamma chain; T3g; Ctg3; Ctg-3
• Gene ID 2: 12502
• mRNA Refseq 2: NM_009850
• Protein Refseq 2: NP_033980