Active Recombinant Mouse CD70 protein, His-Avi-tagged, Biotinylated

• Cat.No.: CD70-2987M
• Product Overview: Biotinylated Recombinant Mouse CD70 protein(Gln 47-Pro 195), fused with His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His-Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse CD27, Mouse IgG2a Fc Tag, low endotoxin at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse CD27 Ligand, His,Avitag with a linear range of 0.1-3 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).The protein has a calculated MW of 20.1 kDa. The protein migrates as 28-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Gln 47-Pro 195
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd70 CD70 antigen [ Mus musculus ]
• Official Symbol: CD70
• Synonyms: CD70; CD70 antigen; CD27-L; CD27 ligand; Ki-24 antigen; tumor necrosis factor ligand superfamily member 7; tumor necrosis factor (ligand) superfamily, member 7; Cd27l; CD27LG; Tnfsf7
• Gene ID: 21948
• mRNA Refseq: NM_011617
• Protein Refseq: NP_035747