Active Recombinant Mouse CD80 Homodimer Protein, Fc tagged

• Cat.No.: Cd80-26M
• Product Overview: Mouse CD80 protein dimer contains the CD80 extracellular domain (UniProt# Q00609) fused with a proprietary cis-dimer motif followed by a Fc tag at the C-terminus was expressed in HEK293T.

Specification

• Species: Mouse
• Source: HEK293T
• Tag: Fc
• Protein Length: 38-246 aa
• Description: Cluster of differentiation 80 (CD80) is a Type I transmembrane glycoprotein in the immunoglobulin superfamily and a member of the B7 Family of ligands. CD80 is also known as B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, and LAB7. CD80 contains an extracellular domain (ECD), a transmembrane domain, and a cytoplasmic domain. The ECD consists of two immunoglobulin (Ig)-like subdomains, a variable-like domain (Ig-V-like domain), and a constant-like domain (Ig-C-like domain). It is primarily expressed on antigen-presenting cells (APCs), such as dendritic cells, macrophages, and B cells. CD80 interacts with CTLA-4 (Cytotoxic T-lymphocyte associated protein 4) to transmit an inhibitory signal with T cells and CD28 (Cluster of differentiation 28) to transmit a stimulatory signal. It is often overexpressed in various autoimmune diseases such as multiple sclerosis and systemic lupus erythematosus, as well as some cancers. CD80 exists as a monomer but its dimeric form can influence immune regulation and contribute to pathogenic conditions. A recombinant protein mimicking the CD80 dimer conformation can be crucial for therapeutic discovery. While structurally and functionally similar to human CD80, mouse CD80 is a species-specific tool essential for preclinical studies, basic research and translational research in cancer immunotherapy.
• Molecular Mass: 100 kDa
• Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
• Application: Verified Applications: ELISA for CD80-specific antibody binding assays and SPR and ELISA for CD80 and CTLA-4 or CD28 ligand protein binding assays. Suggested Applications: SPR & BLI for CD80-specific antibody binding assays and BLI for CD80 and CTLA-4 or CD28 protein binding assays. Animal immunization, RUO.
• Storage: Store at -80 centigrade.
• Storage Buffer: 0.22μm filtered PBS, pH 7.4
• Shipping: Frozen Dry Ice

Gene Information

• Gene Name: Cd80 CD80 antigen [ Mus musculus (house mouse) ]
• Official Symbol: Cd80
• Synonyms: CD80; CD80 antigen; T-lymphocyte activation antigen CD80; B7 protein; activation B7-1 antigen; B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
• Gene ID: 12519
• mRNA Refseq: NM_009855
• Protein Refseq: NP_033985
• UniProt ID: Q00609