Active Recombinant Mouse Cd80 protein(Met1-Lys245), His-tagged

• Cat.No.: Cd80-5171M
• Product Overview: Recombinant Mouse CD80 (Q00609-1) extracellular domain (Met 1-Lys 245) was expressed in HEK293, with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Protein length: Met1-Lys245
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to bind recombinant mouse CD28 in a functional ELISA.
• Molecular Mass: The recombinant mouse CD80 consists of 219 amino acids and has a predicted molecular mass of 25.1 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD80 is approximately 45-55 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd80 CD80 antigen [ Mus musculus ]
• Official Symbol: Cd80
• Synonyms: CD80; CD80 antigen; T-lymphocyte activation antigen CD80; B7 protein; activation B7-1 antigen; B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
• Gene ID: 12519
• mRNA Refseq: NM_009855
• Protein Refseq: NP_033985