Active Recombinant Mouse Ces1d Protein, His-tagged

• Cat.No.: Ces1d-863M
• Product Overview: Recombinant mouse CES3 (NP_444430.2) (Met1-Glu561) was expressed with a C-terminal polyhistidine tag.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-561 a.a.
• Predicted N Terminal: Tyr 19
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its ability to hydrolyze p-nitrophenylacetate.The specific activity is >11000 pmoles/min/μg.
• Molecular Mass: The recombinant mouse CES3 comprises 554 amino acids and has a predicted molecular mass of 60.8 kDa. The apparent molecular mass of the protein is approximately 53 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Ces1d carboxylesterase 1D [ Mus musculus ]
• Official Symbol: Ces1d
• Synonyms: TGH; Ces3; FAEE synthase; carboxylesterase 3; fatty acid ethyl ester synthase; triacylglycerol hydrolase
• Gene ID: 104158
• mRNA Refseq: NM_053200
• Protein Refseq: NP_444430
• UniProt ID: Q8VCT4
• Chromosome Location: 8; 8 C5
• Pathway: Biological oxidations, organism-specific biosystem; Drug metabolism - other enzymes, organism-specific biosystem; Metabolism, organism-specific biosystem
• Function: carboxylic ester hydrolase activity; carboxylic ester hydrolase activity; sterol esterase activity