Ccl21a-412M
Optional Service: Optional requirements on this protein
Product Overview :
Recombinantmouse Ccl21a protein was expressed in E. coli. Ser24-Gly133.
Description :
Mouse Ccl21a cDNAencodes a 133 amino acid residue protein with a 23 residue signal peptide thatis cleaved to generate the 110 residue mature protein. Human and mouse Ccl21aare highly conserved, exhibiting 86% amino acid sequence identity. Ccl21a isconstitutively expressed at high levels in lymphoid tissues such as lymphnodes, spleen and appendix.
Source :
E. coli
Form :
Lyophilized from a0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrierprotein.
N-terminal Sequence :
Ser24
Molecular Weight :
12 kDa
Activity :
Measured by itsability to chemoattract 5-10 day cultured human peripheral blood lymphocytes(PBL). The ED50 for this effect is typically 50-200 ng/mL. Measuredby its ability to chemoattract human CCR7 transfected BaF3 mouse pro-B cells.The ED50 for this effect is typically 3-15 ng/mL.
Endotoxin Level :
<1.0 EU per 1 μgof the protein by the LAL method.
Purity :
>97%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution :
Reconstitute at 10μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin.
Storage :
Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month,2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions afterreconstitution.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.