Active Recombinant Mouse Chemokine (C-C motif) Ligand 25

• Cat.No.: Ccl25-415M
• Product Overview: Recombinantmouse Ccl25 protein was expressed in E. coli. Gln24-Asn144, with anN-terminal Met.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Protein Length: 24-144 a.a.
• Description: Ccl25 is a novel CCchemokine that is distantly related (twenty-some% amino acid sequenceidentity) to other CC chemokines. Mouse Ccl25 cDNA encodes a 144 amino acidresidue precursor protein with a 23 amino acid residue signal peptide that iscleaved to yield a 121 residue mature protein.
• Form: Lyophilized from a0.2 μm filtered solution in PBS with BSA as a carrier protein.
• N-terminal Sequence: Met
• Molecular Weight: 14 kDa
• Activity: Measured by itsability to chemoattract human CCR9 transfected BaF3 mouse pro-B cells. The ED50for this effect is typically 0.1-0.5 μg/mL.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• Purity: >97%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 10μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month,2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions afterreconstitution.

Gene Information

• Gene Name: Ccl25 chemokine (C-C motif)ligand 25 [ Mus musculus ]
• Official Symbol: Ccl25
• Synonyms: Ccl25; chemokine(C-C motif) ligand 25; TECK; CKb15; Scya25; AI852536;A130072A22Rik; OTTMUSP00000025510; OTTMUSP00000025511;OTTMUSP00000025512; OTTMUSP00000025513; C-C motif chemokine 25;thymus-expressed chemokine; small inducible chemokine 25; small induciblecytokine A25
• Gene ID: 20300
• mRNA Refseq: NM_009138
• Protein Refseq: NP_033164
• UniProt ID: Q3V2F3
• Chromosome Location: 8
• Pathway: Chemokine receptorsbind chemokines; Chemokine signaling pathway; Class A/1 (Rhodesian-likereceptors); signaling pathway; Class A/1 (Rhodesian-like receptors); Cytokine-cytokinereceptor interaction; G alpha (i) signaling events; GPCR downstream signaling;GPCR ligand binding; Peptide ligand-binding receptors; Signal Transduction; Signalingby GPCR
• Function: CCR10 chemokinereceptor binding; chemokine activity; cytokine activity; protein binding