Active Recombinant Mouse Csf1 (33-262aa), Met tagged

• Cat.No.: Csf1-041E
• Product Overview: Recombinant Mouse Csf1 (Lys33 ­ Glu262, with an N­terminal Met ) was expressed in E. coli and produced using non­animal reagent in an animal­free laboratory.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Description: M­CSF, also known as CSF­1, is a four­μ­helical­ bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M­CSF is also essential for the survival and proliferation of osteoclast progenitors. M­CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis . M­CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M­CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells.
• N­terminal Sequence Analysi: Met
• Structure / Form: Disulfide­linked homodimer
• Molecular Mass: 26 kDa (monomer)
• SDS­PAGE: 52 kDa, non­reducing conditions
• Activity: Measured in a cell proliferation assay using M­NFS­60 mouse myeloid cells. The ED50 for this effect is typically 0.5 ­ 3 ng/mL.
• Endotoxin Leve: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS­PAGE under reducing conditions and visualized by silver stain.
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS. See Certificate of Analysis for details.
• Reconstitution: Reconstitute at 200 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
• Storage: Use a manual defrost freezer and avoid repeated freeze­thaw cycles. 1. 12 months from date of receipt, ­20 to ­70 °C as supplied. 2. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3. 3 months, ­20 to ­70 °C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Csf1 colony stimulating factor 1 (macrophage) [ Mus musculus ]
• Official Symbol: Csf1
• Synonyms: Csf1; colony stimulating factor 1 (macrophage); op; Csfm; MCSF; C87615; macrophage colony-stimulating factor 1; osteopetrosis
• Gene ID: 12977
• mRNA Refseq: NM_001113530
• Protein Refseq: NP_001107002
• UniProt ID: P07141
• Chromosome Location: 3 F3; 3 51.0 cM
• Pathway: Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; Osteoclast differentiation; Rheumatoid arthritis; Signaling events mediated by PTP1B; Signaling events mediated by TCPTP
• Function: cytokine activity; growth factor activity; macrophage colony-stimulating factor receptor binding; protein homodimerization activity