Active Recombinant Mouse Cst7 Protein, His-tagged

• Cat.No.: Cst7-1061M
• Product Overview: Recombinant Mouse Cst7 (Accession # O89098) Ala19-Gln144, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: Ala19-Gln144
• Predicted N Terminal: Ala19
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl and Brij-35.
• Bio-activity: Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC.The IC50 value is <60 nM, as measured under the described conditions.
• Molecular Mass: Recombinant Mouse Cst7, His-tagged has a calculated MW of 15 kDa. In SDS-PAGE migrates as 20 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Cst7 cystatin F (leukocystatin) [ Mus musculus ]
• Official Symbol: Cst7
• Synonyms: CST7; cystatin F (leukocystatin); cystatin-F; cystatin 7; cystatin-7; cystatin-like metastasis-associated protein; Cmap; MGC151424; MGC151426
• Gene ID: 13011
• mRNA Refseq: NM_009977
• Protein Refseq: NP_034107