| Species : |
Mouse |
| Source : |
HEK293 |
| Tag : |
Fc&His |
| Protein Length : |
Met1-Gly237 |
| Form : |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bio-activity : |
Measured by the ability of the immobilized protein to support the adhesion of mouse neutrophils. When 5 x 10E4 cells/well are added to CXADR-coated plates (4 μg/ml and 100 μl/well), approximately 20%-40% will adhere specifically after 60 minutes at 37°C. |
| Molecular Mass : |
The recombinant mouse CXADR/Fc is a disulfide-linked homodimer. The reduced monomer consists of 466 amino acids and has a predicted molecular mass of 52 kDa. As a result of glycosylation, the apparent molecular mass of rm CXADR/Fc monomer migRates with an apparent molecular mass of 60-65 kDa in SDS-PAGE under reducing conditions. |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
| Purity : |
> 92 % as determined by SDS-PAGE |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
