Cat. No. : |
Crisp4-5612M |
Description : |
Crisp4 is one ofseveral CRISPs (cysteine-rich secretory proteins) that contain 16 invariantcysteine residues and show structural homolgy to snake, insect, and lizardvenom proteins. These proteins consist of an N-terminal SCP/CAP domain, ahinge region, and a cysteine-rich CRISP domain. CRISPs are expressed indiscrete but overlapping regions of the male reproductive tract and play arole in spermatozoa adhesion with Sertoli cells and oocytes, decapacitation,and the acrosomal reaction. |
Source : |
Chinese HamsterOvary cell line, CHO-derived |
Form : |
Lyophilized from a0.2 μm filtered solution in PBS. |
N-terminalSequence : |
Leu23 |
Molecular Weight : |
27.2 kDa |
Activity : |
Measuredby the ability of the immobilized protein to support the adhesion of NTera-2human testicular embryonic carcinoma cells. The ED50 for thiseffect is typically 0.3-1.5 μg/mL after 1 hour incubation at 37 °C. Optimaldilutions should be determined by each laboratory for each application. |
Endotoxin Level : |
<1.0 EUper 1 μg of the protein by the LAL method. |
SDS-PAGE : |
34-37kDa, reducing conditions |
Purity : |
>95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
Reconstitution : |
Reconstituteat 200 μg/mL in PBS |
Storage : |
Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution. |