Active Recombinant Mouse Dickkopf Homolog 4 (Xenopus laevis), His-tagged

• Cat.No.: Dkk4-404M
• Product Overview: Recombinantmouse Dkk4 protein was expressed in Murine myeloma cell line, NS0-derived, with a C-terminal6-His tag.Leu19-Ile221.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: 19-221 a.a.
• Description: Dkk4 is a member ofthe Dickkopf protein family that includes Dkk -1, -2, -3, and -4 and arelated protein, Soggy. Expression of Dkk4 has only been shown with sensitivePCR techniques during early embryonic development in mice, in differentiatedhuman ES cells, or in mice that express dominant-active β-catenin elevatingWnt signaling in the forebrain.
• Form: Lyophilized from a0.2 μm filtered solution in PBS with BSA as a carrier protein.
• N-terminal Sequence: Leu19
• Molecular Weight: 23 kDa
• Activity: Measured by itsability to inhibit proliferation in MC3T3-E1 mouse osteoblastic cells. The ED50for this effect is typically 1-4 μg/mL. Optimal concentrations should bedetermined by each laboratory for each application.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 100μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Dkk4dickkopf homolog 4 (Xenopus laevis) [ Mus musculus ]
• Official Symbol: Dkk4
• Synonyms: Dkk4;dickkopf homolog 4 (Xenopus laevis); Dkk-4; MGC25705; dickkopf-relatedprotein 4; dickkopf-4
• Gene ID: 234130
• mRNA Refseq: NM_145592
• Protein Refseq: NP_663567
• UniProt ID: Q8VEJ3
• Chromosome Location: 8
• Pathway: Wnt signalingpathway