Active Recombinant Mouse ENO2 protein, His-tagged

• Cat.No.: ENO2-0553M
• Product Overview: Recombinant Mouse ENO2 protein(Met1-Met431), fused with His tag, was expressed in E.coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: 1-431 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 0.5 M Arginine, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Mouse NSE, His Tag at 1 μg/mL (100 μL/well) can bind Anti-NSE Antibody with a linear range of 0.004-0.125 μg/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminusThe protein has a calculated MW of 49.3 kDa. The protein migrates as 47-50 kDa under reducing (R) condition (SDS-PAGE).
• Proteinlength: Met1-Met431
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Eno2 enolase 2, gamma neuronal [ Mus musculus ]
• Official Symbol: ENO2
• Synonyms: ENO2; enolase 2, gamma neuronal; gamma-enolase; neural enolase; neuron-specific enolase; 2-phospho-D-glycerate hydro-lyase; NSE; Eno-2; AI837106; D6Ertd375e
• Gene ID: 13807
• mRNA Refseq: NM_013509
• Protein Refseq: NP_038537