Active Recombinant Mouse Epha3 Protein, Fc-tagged, Alexa Fluor 488 conjugated

• Cat.No.: Epha3-494MAF488
• Product Overview: A DNA sequence encoding the extracellular domain of mouse EPHA3 (NP_034270.1)(Met1-His541) (Alexa Fluor 488 conjugated) was expressed with the Fc region of human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: Fc
• Form: Lyophilized
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized mouse EFNA5-His at 10 μg/mL (100 μL/well) can bind mouse EPHA3-Fc, The EC50 of rat mouse EPHA3-Fc is 12.3-28.9 ng/mL.
• Molecular Mass: The recombinant mouse EPHA3/Fc comprises 762 amino acids and has a predicted molecular mass of 85.7 kDa. The apparent molecular mass of the protein is approximately 96 kDa in SDS-PAGE under reducing conditions.
• Protein length: Met1-His541， 762
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 488 via amines
  Excitation Wavelength: 488 nm
  Emission Wavelength: 515­-545 nm
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH7.4.

Gene Information

• Gene Name: Epha3 Eph receptor A3 [ Mus musculus ]
• Official Symbol: Epha3
• Synonyms: EPHA3; Eph receptor A3; ephrin type-A receptor 3; EK4; EPH-like kinase 4; tyrosine-protein kinase TYRO4; tyrosine-protein kinase receptor ETK1; Hek; Cek4; ETK1; End3; Hek4; Mek4; Tyro4; AW492086
• Gene ID: 13837
• mRNA Refseq: NM_010140
• Protein Refseq: NP_034270